Koma BioTech/Pfu DNA聚合酶试剂盒/K0566001/250U
商品编号：
K0566001
品牌：
komabiotech
市场价：
¥0.00
美元价：
0.00
产品分类：
酸碱缓冲液
联系Q Q：
3392242852
电话号码：
4000-520-616
电子邮箱：
info@ebiomall.com
商品介绍
| Description |
|---|
| Pfu DNA polymerase isolated from Pyrococcus furiosus, a proofreading DNA polymerase due to 3'→5'exonuclease activity exhibits the lowest error rate of any thermostable DNA polymerase studied. This feature makes Pfu DNA polymerase the ideal choice for a variety of techniques requiring high fidelity DNA synthesis by PCR such as cloning, gene expression, and site-directed mutagenesis. Pfu DNA polymerase is a highly thermostable enzyme, retaining 94-99% of its polymerase activity after 1 hour at 95°C. To amplify GC-rich regions, denaturation temperature up to 98C can be used to with Pfu DNA polymerase. Pfu DNA polymerase is devoid of terminal deoxynucleotidyltrasferase (TdT) activity, which is characterized by the addition of nontemplate-directed nucleotide(s) at the 3'-end of PCR-generated fragments. Therefore, it generates blunt-ended PCR products. This kit contains all optimized reagents for PCR reaction. |
| Specification |
| • High PCR fidelity with improved yield • Highly thermostable: active more than 4 hrs at 95°C • Source: E.coli, recombinant gene from Pyrococcus furiosus • Lowest error rate • 3'→5' exonuclease activity • No extra A addition activity: Generate blunt-ended PCR products • Amplification range: - Lambda DNA: up to 60Kb - Human genomic DNA: up to 2Kb • Composition of all optimized reagents for PCR reaction (Pfu DNA Polymerase, Pfu PCR buffer containing MgSO4, dNTP mix, loading dye, nuclease free water and Magic buffer) in separate tubes • A supply of enhancer (Magic buffer) for GC rich template or secondary structures |
| Components |
| • Pfu DNA Polymerase • 10X Pfu PCR Buffer • 10X dNTP Mix (2.5mM each) • 10X Loading Buffer • Nuclease Free Water • 4X Magic Buffer |
| Citation |
品牌介绍
Koma BioTechKoma生物技术公司总部设在韩国首尔，生产和提供用于生命科学研究的试剂和工具。综合产品线支持应用于所有主要生命科学研究领域，包括分子生物学、免疫学、细胞生物学、蛋白质组学和基因组学研究。主要产品包括蛋白质电泳系统&预铸凝胶、PCR扩增试剂、转染试剂、直接PCR、核酸纯化试剂盒、细胞因子ELISA试剂盒、免疫球蛋白。KOMA BIOTECH, based in Seoul, Korea, manufactures and provides reagents and tools for life science research. The comprehensive product lines support applications in the study of all major life science research areas, including molecular biology, immunology, cell biology, proteomics and genomics research. Key products include protein electrophoresis systems & pre-cast gels, PCR amplification reagents, Transfection reagent, Direct PCR, nucleic acid purification kits and ELISA kits for cytokines, immunoglobulins.品牌货号名称规格Koma BioTechK0331121HSHuman IFN-gamma High Sensitivity ELISA Kit96wellKoma BioTechK0331800HSHuman IL-1 beta High Sensitivity ELISA Kit96wellKoma BioTechK0331194HSHuman IL-6 High Sensitivity ELISA Kit96wellKoma BioTechK0331216HSHuman IL-8 High Sensitivity ELISA Kit96wellKoma BioTechK0331123HSHuman IL-10 High Sensitivity ELISA Kit96wellKoma BioTechK0331131HSHuman TNF-alpha High Sensitivity ELISA Kit96wellKoma BioTechK0331138HSMouse IFN-gamma High Sensitivity ELISA Kit96wellKoma BioTechK0331230HSMouse IL-6 High Sensitivity ELISA Kit96wellKoma BioTechK0331186HSMouse TNF-alpha High Sensitivity ELISA Kit96wellKoma BioTechK0333000Bovine IL-13 ELISA Kit96well x 2Koma BioTechK0333007Bovine IFN-gamma ELISA Kit96well x 2Koma BioTechK0333002Bovine IL-13 ELISA Kit96well x 2Koma BioTechK0333004Bovine IL-17A ELISA Kit96well x 2Koma BioTechK0333001Bovine MCP-1 ELISA Kit96well x 2Koma BioTechK0333003Bovine MIG ELISA Kit96well x 2
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